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Barcoded ssDNA adapters [IDT for barcode details see were ligated to the fragmented RNA (9 5% DMSO (Sigma) 1 mM ATP 20% PEG8000 and 1 U/μl T4 RNA ligase I (NEB) in 50 mM Tris HCl pH 7 5 (Sigma) 10 mM MgCl 2 and 1 mM DTT 22C for 2 hours) and a second RT reaction (Affinity Script RT buffer 10 mM DTT 4 mM dNTP 2 5 U/μl Affinity Script RT enzyme (Agilent) 2 min at 42C 45 min at
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Mass is a scientific measure of the amount of matter an object is made up of No matter where you are at given moment in time mass is constant So whether you're walking to the shop to stock up on groceries or bouncing around on the moon your mass is the same Obviously if you're on a diet that's not the answer you want to hear but don't panic - we'll get to the all-important definition of
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Volumetrics are the relationships between mass and volume Mass Volume Specific Gravity G Guarinkth se Volumetric Relationships V V sb V a VMA Air V ba V b V se V be Solids Binder Guarinkth se Basic Terms Specific Gravity (G) G xy x b = binder s = stone (i e aggregate) m = mixture y b = bulk e = effective a = apparent m = maximum Example G mm = gravity mixture
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Definition The specific weight γ of a material is defined as the product of its density and the standard gravity g = The density of the material is defined as mass per unit volume typically measured in kg/m 3 The standard gravity is acceleration due to gravity usually given in m/s 2 and on Earth usually taken as 9 81 m/s 2 Unlike density specific weight is not a fixed property
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The diameter of the larger piston is 600 mm and it is required to support a mass of 3500 kg the press is filled with a hydraulic fluid of specific gravity 0 8 Calculate the force required on the smaller piston to provide the required force i When the two pistons are at same level ii When the smaller piston is 2 6 m below the larger piston (536 48 N 627 92 N) f A hydraulic jack has
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- Stone on Stone contact - High permeability * Well graded - Good interlock - Low permeability * Gap graded - Lacks intermediate sizes - Good interlock - Low permeability Aggregate Blending Absorption Specific Gravity 24 100 0 075 3 2 36 4 75 9 5 12 5 19 0 Percent Passing control points max density line max size nom max size Sieve Size (mm) Raised to 0 45 Power Design Aggregate Structure
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You can find metric conversion tables for SI units as well as English units currency and other data Type in unit symbols abbreviations or full names for units of length area mass pressure and other types Examples include mm inch 100 kg US fluid ounce 6'3 10 stone 4 cubic cm metres squared grams moles feet per second and many more!
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Kidney stones can develop in 1 or both kidneys and most often affect people aged 30 to 60 They're quite common with more than 1 in 10 people affected Kidney stones are usually found in the kidneys or in the ureter the tube that connects the kidneys to your bladder They can be extremely painful and can lead to kidney infections or the kidney not working properly if left untreated
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Once the effects of crushed stone dust on concrete properties are obtained it will be possible to take necessary measures to alleviate its adverse effects on concrete Experimental Program Materials In all mixes limestone crushed rock coarse and fine aggregates were used Dust passing 75 um BS sieve was produced in a laboratory crusher The maximum size of coarse aggregate was 28 mm The
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Stone or ceramic flooring is preferred although some manufacturers make vinyl and wood that is efficient and safe to use with underfloor heating It is argued that underfloor heating is energy efficient because it allows hot air to rise naturally from the floor through the room and the temperatures that are generally needed for comfort are several degrees lower than those needed for rooms
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Volumetrics are the relationships between mass and volume Mass Volume Specific Gravity G Guarinkth se Volumetric Relationships V V sb V a VMA Air V ba V b V se V be Solids Binder Guarinkth se Basic Terms Specific Gravity (G) G xy x b = binder s = stone (i e aggregate) m = mixture y b = bulk e = effective a = apparent m = maximum Example G mm = gravity mixture
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Thin stone wall systems used for exterior building envelopes typically consist of stone panels ranging in thickness from 3/4 inches to 2 inches Most panels are fabricated from granite while marble limestone travertine and sandstone are also used to a lesser extent A common panel thickness is 1-3/16 inch (3 cm) Overall panel dimensions can vary significantly for different buildings
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Learn About Stone Locations Our Locations FAQ We Have the Answer Job of the Month Our Monthly Contest Promotions View Special Promotions E-Club Join for Special Offers Contact Us Talk to Our Staff The average weight of a sandstone boulder is approximately 150 pounds per cubic foot Limestone boulders and granite boulders in most cases weigh more They average about 175 pounds per cubic
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The stone or stone weight (abbreviation st ) is an English and imperial unit of mass now equal to 6 35 kg (14 pounds) England and other Germanic-speaking countries of northern Europe formerly used various standardised stones for trade with their values ranging from about 5 to 40 local pounds (roughly 3 to 15 kg) depending on the location and objects weighed
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Metric conversion charts and calculators for metric conversions Temperature Weight Length Area Volume Speed Time Angle Pressure Energy and Power Health and Wellbeing The metric system originated in France in 1799 following the French Revolution although decimal units had been used in many other countries and cultures previously
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Product calculator Calculate weight of sand gravel topsoil required by inputting area and optionally get a quote The calculated amount of the sand gravel or topsoil you will need is shown below (we supply our landscaping products in tonnes so our customers get exactly what they ordered)
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4 mm and d greater than or equal to 2 mm Fine Aggregate designation given to the smaller aggregate sizes with D less than or equal to 4 mm Fines particle size fraction of an aggregate which passes the 0 063 mm sieve Grading particle sized distribution expressed as the percentage by mass passing a specified set of sieves
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The use of balances or scales recording pounds of mass (lbm) or the recording of density in lbm/ft 3 should not be regarded as nonconformance with this standard 1 6 3 The sieve designations are identified using the "standard" system in accordance with Specification E11 such as 25 mm and 75 μm followed by the "alternative" system of 1 in and No 200 respectively
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Stone (Sandstone) 2000 0 900 1800 FC Sheet (compressed) 1700 0 900 1530 Earth Wall (Adobe) 1550 0 837 1300 Rammed Earth 2000 0 837 1673 Compressed Earth Blocks 2080 0 837 1740 Table 1 Density specific heat and thermal mass of a range of materials Note Figures are based on a number of sources and include estimations and interpolations 2 3 Thermal comfort Thermal comfort
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Barcoded ssDNA adapters [IDT for barcode details see were ligated to the fragmented RNA (9 5% DMSO (Sigma) 1 mM ATP 20% PEG8000 and 1 U/μl T4 RNA ligase I (NEB) in 50 mM Tris HCl pH 7 5 (Sigma) 10 mM MgCl 2 and 1 mM DTT 22C for 2 hours) and a second RT reaction (Affinity Script RT buffer 10 mM DTT 4 mM dNTP 2 5 U/μl Affinity Script RT enzyme (Agilent) 2 min at 42C 45 min at
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Aggregates for Use In Concrete 2 Learning Objective • Develop a basic understanding of aggregates and aggregate properties 2/12/2015 2 3 Aggregates • Fine Consists of natural sand manufactured sand or crushed stone 3/8" Fine aggregate will pass the # 4 sieve • Coarse Natural or crushed stone 3/8" to 1 " (or more) Coarse aggregate is larger than a #4 sieve 4 Mineralogy
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The density measured is the bulk density d b The total mass then becomes Total mass = V T d b = V T d f + (1- ) V T d ma So we have a formula giving the Mixing Law for density which represents bulk density in terms of the porosity pore fluid density and the matrix density d b = d f + (1-) d ma By re-arranging the terms the porosity can be represented in the following way =(d ma- d b
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